Scintillation Nanotechnologies
nanoSPA and nanoSPA-LA
Next generation scintillation proximity assay materials designed for specific radiolabeled compound detection in aqueous samples, nanoSPA and nanoSPA-LA offer several advantages over existing scintillation proximity materials due to their small size, intermediate density, and versatile surface.
Product Features
Dispersibility and Suspension
The small particle size, low density, and surface chemistry make nanoSPA and nanoSPA-LA readily dispersible and stay in suspension longer than conventional SPA products.
Consistent Signal
A consistently high signal to noise ratio and better reproducibility give you confidence in your data and eliminate the time, cost, and frustration of repeating experiments.
Reduced Non-Specific Binding
nanoSPA-LA is specially formulated to reduce non-specific adsorption allowing highly specific, reproducible measurements in previously impossible applications.
Surface Modifications
The surface chemistry of nanoSPA and nanoSPA-LA allows for a wide range of functionalities to account for commonly available experimental protocols, such as protein A, protein G, streptavidin and his-tagged proteins.
Application Notes
Binding Kinetics
Attach your protein of interest to nanoSPA via a histidine tag and monitor the binding of radiolabeled ligands over time in the same vial or multi-well plate
IC50/EC50
Determine IC50/EC50 or perform other competitive assays for your receptor with sensitivity and reproducibility.
Membrane Protein
Attach detergent solubilized membrane proteins to nanoSPA to maintain protein function.
Build-your-own Assay
nanoSPA is available with protein A, protein G, streptavidin, and functionalized for his-tagged proteins.
Our Technology Platform
Due to their unique chemical and physical properties, nanoSPA and nanoSPA-LA offer unparalleled capabilities in both routine and demanding SPA applications
Our proprietary scintillation proximity assay products were specifically developed to overcome the shortcomings of existing SPA products.
Silica Surface
A silica surface makes the nanoparticles easily dispersible in water and provides an ideal platform for surface functionalization.
Proprietary Core
Our proprietary core delivers consistent, reproducible scintillation results.
Size and Density
Their small size and intermediate density mean our materials stay in suspension longer than competing products.
Versatile Surface
A versatile surface enables a wide range of applications. Current surface functionalizations include Protein G, Protein A, Biotin, and for his-tagged proteins.
Product Guidelines
Instruments and Detectors
nanoSPA is compatible with most PMT-based scintillation counters (blue variants), and CCD-based instruments (red).
Scintillation Counts
nanoSPA is a polymer-based scintillator and will yield lower total counts per unit of radioactivity in most experiments than scintillation cocktail or inorganic crystal-based scintillators.
Usage
nanoSPA can be used in scintillation vials or multi-well plates.
Organic Solvent Compatibility
nanoSPA is not compatible with many organic solvents including acetone, ethyl acetate, toluene, benzene, dimethylsulfoxide, and acetonitrile.
Ordering Information
Product Version | Surface Modification | Color |
nanoSPA |
Streptavidin |
Blue |
nanoSPA |
His-Tagged |
Blue |
nanoSPA |
Protein A |
Blue |
nanoSPA |
Protein G |
Blue |
nanoSPA |
Streptavidin |
Red* |
nanoSPA |
His-Tagged |
Red* |
nanoSPA |
Protein A |
Red* |
nanoSPA |
Protein G |
Red* |
nanoSPA-LA |
His-Tagged |
Blue |
nanoSPA-LA |
His-Tagged |
Red* |
*Red products are available by custom order and will increase lead time. Please contact us below to place an order for red product variants.
Product Resources
His-Tagged nanoSPA Product Sheet
Product Specifications
Compatible Isotopes
-
3H, 14C, 22Na, 32P, 33P, and 35S
Wavelengths
Red approximately 615nm
Blue approximately 430nm
Particle Size
Approximately 300nm
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